Hsa_circRNA_102682 is closely related to lipid metabolism in gestational diabetes mellitus

Abstract

Objective

To explore the relationship between circular RNA (circRNA) in gestational diabetes mellitus (GDM) and the metabolic profile at the molecular level, and find a biological marker that can predict GDM early.

Methods

A retrospective case–control study was conducted using data and samples from women treated at a hospital in China between January 10 2018 and February 20 2019. Reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate the expression level of hsa_circRNA_102682 in serum and analyze its correlation with lipid metabolism parameters.

Results

Advanced age and higher pre-pregnancy body mass index (BMI) during pregnancy are risk factors for GDM. The expression level of hsa_circ_102682 was lower among the cases than the controls (p=.000). The levels of triglyceride, apolipoprotein A1 (APOA1), APOB, and high-density lipoprotein cholesterol (HDL-C) were different between the controls and cases (p<.05). Hsa_circRNA_102682 was significantly correlated with triglycerides, APOA1, APOB, 1-h blood glucose in the serum of GDM patients, and the correlation coefficients were 0.319, 0.314, 0.286, and 0.311, respectively (p<.05). The area under the receiver operating characteristic curve is 0.684 (95% confidence interval 0.611–0.756, p=.0001).

Conclusions

Hsa_circRNA_102682 may regulate lipid metabolism, participate in the pathogenesis of GDM. It can be used as a marker to predict GDM.

Hsa_circRNA_102682与妊娠期糖尿病血脂代谢密切相关 摘要

目的：探讨妊娠期糖尿病(GDM)中环状RNA (circRNA)在分子水平上与代谢谱的关系, 寻找一种能早期预测GDM的生物学标志物。

方法：采用2018年1月10日至2019年2月20日期间在中国一家医院接受治疗的妇女的数据和样本, 开展了一项回顾性病例对照研究。采用反转录聚合酶链反应(qRT-PCR)评价hsa_circRNA_102682在血清中的表达水平, 并分析其与脂质代谢参数的相关性。

结果：高龄和妊娠期较高的孕前体重指数(BMI)是妊娠期糖尿病的危险因素。hsa_circ_102682在病例组中的表达水平低于对照组(p=.000)。对照组与病例组的甘油三酯、载脂蛋白A1 (APOA1)、APOB和高密度脂蛋白(HDL-C)水平差异有统计学意义(p< 0.05)。Hsa_circRNA_102682与GDM患者血清甘油三酯、APOA1、APOB、1h血糖显著相关, 相关系数分别为0.319、0.314、0.286、0.311 (p < 0.05)。受试者工作特征曲线下面积为0.684(95%置信区间0.611-0.756,p=.0001)。

结论：Hsa_circRNA_102682可能调控脂质代谢, 参与GDM的发病机制。它可以作为预测GDM的一个标志。

Keywords:

• Hsa_circRNA_102682
• circular RNA
• gestational diabetes mellitus
• lipid metabolism
• biomarker

Author contributions

All authors were responsible for study conception, design, and funding. YZ supervised the study. HW obtained the data. XZ and HW performed the data analysis and interpretation. JS provided clinical expertise for the analysis. HW and YL drafted the manuscript and JS, MY made critical revisions for intellectual content. All authors approved the final version of the manuscript and agreed to be accountable for all aspects of the work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Ningbo Public Welfare Technology Plan Project [2019C50091 to YZ].