Human adipose tissue-derived MSCs improve psoriasis-like skin inflammation in mice by negatively regulating ROS

Abstract

Background

Psoriasis is chronic incurable skin inflammation. The anti-inflammatory properties of mesenchymal stem cells (MSCs) have been put forward to be involved in several inflammatory diseases. However, little was known about the role of human adipose tissue-derived stem cells (hAD-MSCs) in psoriasis.

Objective

We sought to explore the feasibility of using hAD-MSCs infusion as a therapeutic approach in psoriatic mice.

Methods

We constructed the psoriasis-like model by IMQ implication, treated with hAD-MSCs by subcutaneous injection. To evaluate the efficacy, we examined the histology, CD45 and ROS positive cells by HE and flow cytometry respectively. We also tested the key cytokines with PCR. Moreover, to achieve a better therapeutic effect, we treated the model by combing with vitamin E application.

Results

We found that the classic histological symptoms of psoriasis were relieved after treatment with hAD-MSCs, also, the splenic index, the infiltration of immune cells and several pro-inflammatory cytokines were decreased. Interestingly, we also found that hAD-MSCs could inhibit ROS generation. Moreover, the combination therapy of hAD-MSCs and vitamin E could promote the curative effect with greater ROS inhibition.

Conclusion

These results suggested that hAD-MSCs could be useful for treating psoriasis by negatively regulating ROS.

Keywords:

• Adipose tissue-derived mesenchymal stem cells
• psoriasis
• ROS

Acknowledgment

The author thanks the authorities of Nanjing Medical University for their support. The author has not received funding or writing assistance for this manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).