Analysis of the effect of concentration and magnetic field on bioactivity of humic acids from position of open non-equilibrium systems

ABSTRACT

On the example of cultures of bacteria Pseudomonas sp. and Bacillus sp. the effect of humic acids in the concentration range (0–15 mg/L) on the viability of these bacteria was studied. Multidirectional effects of humic acids on microorganisms were found, namely, at concentrations of 1 and 5 mg/L, they reduce, and at 15 mg/L, they do not affect the survival of the studied cultures of bacteria. It has been established that the treatment of aqueous solutions of humic acids with a weak magnetic field affects their physicochemical properties and structural parameters, as well as enhances the biological effect in relation to different groups of microorganisms. It was established that the multidirectionality of the biological effect of humic acids on microorganisms is well explained from the position of considering humic acids as complex open non-equilibrium systems with a developed system for energy dissipation, which leads to the formation on their basis of diverse and complex self-organized structures with different physicochemical properties. The obtained concentration dependences confirm the possibility of the existence of humic acids in the studied concentration range in at least two states. Moreover, it is the state of the system (the level of self-organization), and not the relationship with the source of origin of a humic acids, mainly determines its properties. This fact determines the possibility of using the concentration of humic acids to establish the level of organization of the system, which will allow to manage the structural organization of these objects and predict their properties.

GRAPHICAL ABSTRACT

KEYWORDS:

• Humic acids
• humic substances
• magnetic field
• bioactivity
• self-organized structures

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The data that support the findings of this study are available on request from the corresponding author, S.O. Dolenko.