Di-n-octyl phthalate degradation by a halotolerant bacterial consortium LF and its application in soil

ABSTRACT

Di-n-octyl phthalate (DOP), a plasticizer used in many different industrial products, is a frequently observed pollutant in the environment. Biodegradation by microorganisms is considered to be a realistic choice for the remediation of DOP contamination. In the present research, the halotolerant bacterial consortium (LF) enriched in our previous research was used to degrade DOP. It was found that the optimal conditions for LF to degrade DOP was temperature 30^oC, pH 6.0, inoculum size >5%, and salt content <3%. LF could degrade a high concentration of DOP (2000 mg/L) with the removal efficiency of 96.33%. Substrate inhibition analyses indicated that the inhibition constant, maximum specific degradation rate and half-saturation constant were 2544.6 mg/L, 0.7 d⁻¹ and 59.1 mg/L, respectively. Based on the analysis of the gas chromatography-mass spectrometry (GC-MS), the biodegradation pathway for DOP by LF was proposed. Furthermore, LF could degrade DOP in soil (100 mg/kg) with the highest removal efficiency of 89.3%. This study is the first report on DOP biodegradation by bacterial consortium. These results suggest that LF can be used to remediate DOP-contaminated environment.

GRAPHICAL ABSTRACT

KEYWORDS:

• PAEs
• di-n-octyl phthalate
• biodegradation
• biodegradation pathway
• soil

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by National Natural Science Foundation of China: [grant number 51704093,51804353]; Open Funding Project of National Key Laboratory of Human Factors Engineering: [grant number 614222207041813, SYFD180051810K]; Science and technology development project of Henan Province: [grant number 182102311007]; First-class disciplines innovation team training projects in Henan University: [grant number 2018YLTD16].