Simultaneous detection of mercury (II), lead (II) and silver (I) based on fluorescently labelled aptamer probes and graphene oxide

ABSTRACT

We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg²⁺, Pb²⁺ and Ag⁺ based on fluorescently labelled nucleic acid aptamer probes and graphene oxide (GO). By this method, three nucleic acid aptamer probes (P_Hg, P_Pb, P_Ag) were designed. The carboxyl fluorescein (FAM), tetramethyl-6-carboxyrhodamine (TAMRA) and cyanine-5 (Cy-5) were respectively selected as fluorophore of aptamer probes, and GO was chosen as quencher. In general, these probes were on free single-stranded state and adsorbed on the surface of GO via π-π interactions, which brought fluorophores of probes and GO into close proximity. Due to the fluorescence resonance energy transfer occurred between fluorophores and GO, the fluorescence was quenched and fluorescence signals were all weak. Under the optimal condition, fluorescence intensities of three fluorophores exhibited a good linear dependence on corresponding ions concentration. The detection limit for Hg²⁺, Pb²⁺ and Ag⁺ were 0.2, 0.5 and 2 nmol/L (3σ, n = 11). Average recoveries of this method were 97.56–104.92%, which indicated the method had a high accuracy and low detection limit. In addition, this proposed method has good selectivity, and there was no crosstalk effect among these probes.

GRAPHICAL ABSTRACT

KEYWORDS:

• Fluorescence
• simultaneous detection
• aptamer
• graphene oxide
• metal ions

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the National Science Foundation of China (21465010), the Open Foundation of Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province (PKLHB1918, PKLHB1730), Hubei Provincial Natural Science Foundation (2018CFB247) and Special Funds for ‘Double First-Class’ Construction in Hubei Province.