Chemical profile, in vitro pharmacological activity and Satureja cuneifolia Ten. evaluation of essential oil based on distillation time

ABSTRACT

The medicinal plant Satureja cuneifolia Ten. was widely utilized as spice, tea and traditional medicine. The objective of the current study was to examine the chemical composition and in vitro biological activities (LOX, MMP-1, and MMP-12 enzyme inhibition activity and cytotoxicity on A549 cell line) of Satureja cuneifolia extracts and essential oils. The essential oils of the flowering aerial parts were hydro-distilled at four different distillation times (5, 30, 60, and 180 min) using the Clevenger apparatus. The total essential oil and four fragments were compared in terms of the major component, yield, and distillation time. Volatile compounds of the infusion were extracted by using HS-SPME. Ethanolic extract had the strongest inhibition activity on the LOX enzyme (84.50%), while the essential oils exhibited more cytotoxic activity on the A549 cell line than the extracts. The oils and the infusion were analyzed using GC-MS and the primary chemicals identified by LC-MS/MS.

KEYWORDS:

• Satureja cuneifolia
• essential oil
• biological activities
• GC-MS/FID
• LC-MS/MS

Acknowledgements

A part of the study was presented as a poster presentation at 50th ISEO 2019–50th International symposium on essential oils, Vienna, Austria, 9–12 September, 2019 and 24th BİHAT 2022–XXIV. Herbal medicine raw materials meeting, Ankara, Turkiye, 23–26 June, 2022.

Disclosure statement

All authors declare that there is no material or other substantive conflict of interest in their article that could affect the results or comments.

Additional information

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.