Tetraclinis articulata essential oil emulsion use as alternative to chemical fungicide to control tomato grey mould disease

ABSTRACT

Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC₅₀ of free radical-scavenging activity and H₂O₂ content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes.

KEYWORDS:

• Antioxidant activity
• Arar tree essential oil
• Gene expression
• Grey mould
• Solanum lycopersicum

Disclosure statement

No potential conflict of interest was reported by the author(s).

Abbreviation list

APX	=	Ascorbate peroxydase
B. cinerea	=	Botrytis cinerea
CAT	=	Catalase
CHI	=	Chitinase
EO	=	Essential oil
ERF	=	Ethylene response factor
FG	=	Fongicide
FM	=	Fresh matter
GAE	=	Equivalent acide gallique
GC-MS	=	Gas chromatography coupled with mass spectrometry
GPOX	=	Guaïacol peroxydase
HPI	=	Hour after inoculation
IC50	=	Inhibition concentration at 50%
LOX	=	Lipoxygénases
MW	=	Molecular wight
PCR	=	Polymerase chain reaction
ROS	=	Reactive oxygenated species
RP-HPLC-DAD-ESI-MSn	=	Chromatographie liquide en phase inverse couplée à la spectrométrie de masse par électro-nébulisation
SOD	=	Superoxyde dismutase
T. articulata	=	Tetraclinis articulata
TAEO	=	Tetraclinis articulata essential oil
TRX	=	Thioredoxine

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.