ABSTRACT
Tripleurospermum callosum (Boiss. & Heldr.) E. Hossain was recorded in Turkish ethnobotanical data for its use against urinary and respiratory system ailments. Infusion, decoction and 96% ethanol extracts of T. callosum aerial parts were prepared for in vitro antimicrobial activity against urinary system pathogens Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Klebsiella aerogenes ATCC 1348 and Candida albicans ATCC 10231. The non-toxic concentrations of extracts and in vivo antimicrobial assay were performed using C. elegans. The extracts were analysed by Liquid Chromatography Mass Spectrometry (LC-MS/MS) for phytochemical composition. The water extracts were non-toxic at between 5000 and 312 µg/mL, while 96% ethanol extract at 312 µg/mL for C. elegans. The infusion extract showed in vivo anti-infective effect 5000–312 μg/mL against Gram-negative strains. The results indicate a potential role of plant extracts with relatively non-toxic and anti-infective effects against urinary system pathogens.
Acknowledgements
This work was financially supported by the Scientific and Technological Research Council of Türkiye (Tübitak) with the Project number : 218S812. The authors thank the Sanovel Pharmaceutical Industry İstanbul, Türkiye, for providing standard antimicrobial agents. We also thank Prof. Dr. Volkan Aksoy for the English proofreading of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Author Contribution
GG: Supervision, project administration, Resources, experimental, methodology, data analysis, writing - original draft, writing - review and editing. DY: Experimental, methodology, software, Data interpretation, writing - original draft, writing - review. FG: Experimental, methodology, data analysis, writing - original draft, writing-review. YBK: resources, writing - review and editing. FD: writing-review and editing. The authors read and approved the final manuscript.