Effect of mate tea (Ilex paraguariensis) on the expression of the leukocyte NADPH oxidase subunit p47^phox and on circulating inflammatory cytokines in healthy men: a pilot study

Abstract

Increased superoxide production by phagocytic NADPH oxidase has been associated with inflammatory conditions. Growing evidences suggest that dietary polyphenols may modulate the expression of NADPH oxidase subunits. Herein, we examined whether soluble mate tea (SMT) consumption – a polyphenol-rich beverage – affects the expression of the leukocyte NADPH oxidase protein p47^phox and/or circulating biomarkers of inflammation and antioxidant biomarkers in humans. In a two-phase study, nine men were requested to drink water (control) for 8 d and then follow a second 8-d period drinking SMT. Blood samples were analysed for p47^phox protein in CD16⁺/CD14⁻ cells, interleukin (IL)-1β (IL-1β), tumour necrosis factor-alpha (TNF-α), IL-6, total phenols, and reduced and oxidised glutathione (GSH and GSSG, respectively) after each study phase. After SMT intake, CD16⁺/CD14⁻ cells' p47^phox protein and serum TNF-α and IL-6 levels were significantly attenuated (P < .05) while plasma phenolic compounds and blood GSH:GSSG ratio were significantly enhanced (P < .05). Consumption of SMT favourably affected leukocytes' p47^phox expression and inflammatory cytokine and antioxidants levels in peripheral blood, which may help decrease oxidative stress and low-grade inflammation.

Keywords:

• Ilex paraguariensis
• mate tea
• polyphenols
• leukocytes
• NADPH oxidase
• cytokines

Acknowledgments

The authors thank Leao Alimentos e Bebidas Co for providing the lyophilised instant mate tea.

Disclosure statement

No potential conflict of interest was reported by the authors. The authors also declare that Leao Alimentos e Bebidas Co. partially supported this study providing the lyophilised instant mate tea.