Potential prebiotic effect of a long-chain dextran produced by Weissella cibaria: an in vitro evaluation

Abstract

Dextrans are homopolysaccharides of D-glucose units produced by lactic acid bacteria. They have several technological applications and potential utilisation in positively modulating gut microbiota is attracting increasing attention. Whereas the prebiotic activity of low polymerisation degree (DP) dextrans has been established, high DP dextrans still deserve deeper investigation. In the present study, a long linear chain dextran produced by Weissella cibaria was compared to inulin with regards to the growth of specific health-related taxa and to the production of organic acids in pH-controlled batch cultures of intestinal microbiota. qPCR quantification of Lactobacillus, Bifidobacterium, Prevotella, Bacteroides fragilis, and Faecalibacterium prausnitzii revealed differences in their relative abundance, depending on the carbon source, that reflected the pattern of fermentation products determined by HPLC. Dextran mainly enhanced the relative amount of Prevotella and Bacteroides, consistently with a favourable acetate–propionate ratio suggesting a promising utilisation as functional ingredient in the food industry.

Keywords:

• Extracellular polysaccharides
• dextran
• Weissella cibaria
• faecal microbiota
• short-chain fatty acids
• prebiotic

Acknowledgements

The authors are grateful to BIO-e.r.g. S.r.l. for having provided the NEXTDEXT^® dextran. The authors are also grateful to PhD Alessia Levante for having performed statistical analysis on real-time PCR data.

Disclosure of interest

The authors report no conflict of interest.