Protein crosslinking: Uses in chemistry, biology and biotechnology

Abstract

Protein crosslinking is a part of many biological processes and is also carried out in vitro under several controllable conditions with the help of any of the commercially available bifunctional reagents. Many biotechnological applications utilize stable and/or reusable crosslinked enzymes such as soluble intramolecularly crosslinked enzymes; soluble bioconjugates of enzymes with other enzymes/proteins or polymers; chemically aggregated enzymes, chemically crosslinked enzyme aggregates and crosslinked enzyme crystals. The review after indicating how protein crosslinking is at the heart of such diverse processes/technology concludes with discussion of few applications which are currently drawing considerable attention.

Keywords:

• Crosslinking reagents
• CLEA
• CLEC
• protein crosslinking
• glutaraldehyde
• protein precipitation
• protein aggregation
• protein immobilization

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The financial support provided by University Grant Commission (UGC) Government of India to MP is greatly acknowledged.