Organic N compounds in plant nutrition: have methodologies based on stable isotopes provided unequivocal evidence of direct N uptake?

ABSTRACT

During the past two decades, interest has developed in regard to the possibility that plant roots can take up organic N compounds directly, a concept which challenges the conventional wisdom that soil inorganic N forms (${\mathrm{NH}}_4^{+}$ and ${\mathrm{NO}}_3^{-}$) are the sole primary sources of N absorbed by plant roots. We reviewed the literature based on single or dual (¹⁵N, ¹³C) stable isotope labelling techniques to test the hypothesis of direct uptake. Both isotopically enriched and natural abundance approaches were reviewed. Of the methods examined, the dual enrichment technique, when combined with compound specific and position-specific stable isotope analysis, provided incontrovertible evidence for direct uptake of simple amino acids. We demonstrate that dual labelling lacks overall sensitivity due to the high C concentration in plant tissue relative to N, and the higher natural abundance of ¹³C cf. ¹⁵N, which limits the period of measurement due to isotope dilution, and hence an assessment of the long-term contribution of direct uptake to the N economy of plant communities.

KEYWORDS:

• Carbon-13
• carbon-14
• isotope ecology
• nitrogen-15
• nitrogen uptake
• plant nutrition
• amino acids
• glycine
• ammonium
• nitrate

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by CSIRO Agriculture and the Faculty of Veterinary and Agricultural Sciences, University of Melbourne.