![Sample our Food Science & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5936/TOC-Subject-Sample-2021-Food-Science-Technology.jpg"})
ABSTRACT
Objectives
The study was aimed to evaluate the efficacy of three commonly consumed Lactuca sativa (LS) Linn. varieties viz., Grand rapid, Lollo rosso and Iceberg (Asteraceae) against 3-NP induced HD like symptoms in rats.
Methods
Ethanol extracts of leaves of three LS varieties were prepared, and standardized on the basis of quercetin content using HPLC. These extracts (100 and 200 mg kg, p.o. for 20 days) were evaluated for their neuroprotective effect against 3-NP (10 mg/kg, i.p. for 14 days) induced neurotoxicity in male Wistar rats. The extract that exhibited maximum activity was successively fractionated using hexane, ethyl acetate, n-butanol and aqueous in increasing order of polarity. These fractions were also evaluated (dose equivalent to the dose of the extract of LS variety exhibiting maximum activity) for their neuroprotective effect. The protective effect of extracts and fractions was evaluated using different behavioral (rota rod, actophotometer, beam walk and Morris water maze) and biochemical (malondialdehyde, nitrite, superoxide dismutase, catalase and reduced glutathione) parameters.
Results
3-NP elicit marked deterioration in motor coordination, locomotor activity and memory in comparison to control group. Standardized ethanol extract of grand rapid (200 mg/kg) exhibited maximum activity amongst the three tested varieties. Therefore, its fractions were also evaluated, and n-butanol fraction (40 mg/kg) exhibited maximum attenuation of 3-NP induced HD like symptoms which was evident from improved behavioral and biochemical parameters.
Discussion
The results exhibit that LS (Grand rapid variety) prophylaxis mitigated 3-NP induced neurotoxicity and HD like symptoms in rats due to its potent antioxidant potential.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Additional information
Funding
Notes on contributors
Jai Malik
Dr. Jai Malik is working as Assistant Professor of Pharmacogonsy at University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India. He has been working in the field of standardization of herbal drugs, bioactivity guided fractionation and isolation of bioactive compounds from plants with special reference to the compounds/plants for neurodegenerative disorders like Alzheimer's disease and Huntington's disease. He is also working in the area of phytochemical analysis and development of herbal formulations for such disorders. He has various publications in national and international journals of repute and two books (one in press) to his credit. At present he is working on a project of development of a polyherbal formulation with nano-delivery system for memory disorders.
Supreet Kaur
Ms. Supreet Kaur did her Masters of Pharmacy in Pharmacognosy under guidance of Dr. Jai Malik and Prof. Maninder Karan at University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh. At present she is working as an Associate Medical Writer in Parexel International, Chandigarh.
Maninder Karan
Prof. Maninder Karan is working as Professor of pharmacognosy at University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India. She has been working in the field of natural products isolation, drug discovery & development, standardization & quality control and formulation development of herbal drugs and crystal engineering of natural molecules. She has more than 100 publications to her credit. She has filed 6 patents and granted one. She has transferred technology of Darvi Gel preparation for leucorrhea which has been incorporated in Ayurvedic Pharmacopoeia of India as a propriety medicine. She has also been involved in development of herbal drug monographs for Ayurvedic Pharmacopoeia of India and ICMR, New Delhi.
Sunayna Choudhary
Ms. Sunayna Choudhary did her Masters of Pharmacy in Pharmacognosy from ISF college of Pharmacy, Moga, Punjab under guidance of Dr. Jai Malik. At present she is pursuing her doctorate at University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India, under guidance of Dr. Jai Malik. She has presented her work at national and international platforms and has won best paper awards.