Abstract
A phytochemical investigation on the 80% ethanol extract of the roots of Caragana stenophylla Pojark. resulted in the isolation of 20 compounds, including two new ones, named kompasinol P (2) and 3,5,7,2',3'-pentahydroxy-4'-methoxyisoflavanone (3). Among them, a pair of enantiomers, (7S, 8 R, 7′R, 8′S)-kompasinol A (1a) and (7 R, 8S, 7′S, 8′R)-kompasinol A (1b), were successfully separated by the chiral-phase HPLC resolution for the first time. The absolute configurations of 1a and 1b were determined by the experimental and calculated electronic circular dichroism (ECD) data. 15 isolates were evaluated for their anti-inflammatory activity via inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Compounds 1a/1b, 6, 7, 9, 10, 12, 14, and 16–18 showed moderate inhibition with IC50 values ranging from 11.45 to 68.54 μM.
Graphical Abstract
![](/cms/asset/f54f0f73-5a56-4cd7-89d9-042ca8d5100b/ganp_a_1918119_uf0001_c.jpg)
Disclosure statement
No potential conflict of interest was reported by the author(s).