Structure elucidation and NMR spectral assignments of one new dammarane-type triterpenoid saponin from black ginseng

Abstract

New dammarane-type triterpenoid saponin, named 22(R)-notoginsenoside Ab1 (1), together with thirteen known dammarane-type triterpenoid saponins (2-14) was isolated from the EtOH extract of black ginseng and their structures were elucidated on the basis of one- and two-dimensional NMR (including ¹H-NMR, ¹³C-NMR, HSQC, HMBC, ROESY) and calculated ECD. Among them, compounds 1-2 and 6-8 were isolated for the first time from ginseng and black ginseng. Besides, the absolute structure of 22(R)- and 22(S)- notoginsenoside Ab1 were distinguished by ECD for the first time.

Keywords:

• NMR
• ECD
• triterpenoid saponin
• notoginsenoside
• ginsenoside
• black ginseng

Acknowledgments

The authors gratefully appreciate Professor Hui-Ming Hua and her student Meng-Yue Yang for their kind help in ECD calculation.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors gratefully acknowledge the support of the National Nature Science Foundation of China (grant no. 82073985) and the Key Research and Development Plan of Liaoning Province (grant no.2020JH2/10300055).