Synthesis, α-Glucosidase and β-Galactosidase Inhibitory Potentials and Molecular Docking of Some Novel Benzofuran-Pyridazine Derivatives

Abstract

A novel series of benzofuran-pyridazine derivatives have been synthesized and characterized by different spectroscopic techniques including FT-IR, ¹H-NMR, ¹³C-NMR, and ESI-MS spectrometry. All synthesized compounds were evaluated in vitro for their antidiabetic activity against α-glucosidase and β-galactosidase enzymes. All derivatives (3a-3h) and (4a-4b) showed a remarkable inhibitory potential greater than 89% against α-glucosidase enzyme compared to standard acarbose (42.50%), and compounds 3 b and 4a exhibited significant inhibitory potential against β-galactosidase enzyme with 50.33% and 57.67% respectively, compared to standard Queretin (52.00%). A molecular docking study was conducted to understand the binding interactions of the compounds with the active site of the α-glucosidase enzyme.

Keywords:

• Benzofuran
• pyridazin-3(2H)-one
• pyridazin-3(2H)-thione
• α-glucosidase
• β-galactosidase
• molecular docking

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work is supported by UM5R. The authors would like to acknowledge the UATRS-CNRST for NMR and mass spectra.