Recent Advances in Capillary Electrochromatography of Proteins and Carbohydrates in the Biopharmaceutical and Biomedical Field

Abstract

Capillary electrochromatography (CEC) is a powerful hybrid separation technique that combines capillary electrophoresis and capillary chromatography, capable to address the analytical challenges of proteomics and glycomics. The focus of this paper is to review the recent developments in capillary electrochromatography of proteins and carbohydrates. The different column types applied in capillary electrochromatography such as packed bed, open tubular and monoliths are conferred in detail with respective separation examples. A comprehensive comparison is also given listing the mostly utilized coating methods, stationary phase materials and column preparation methods. The choice of porogenic solvent combinations for monolithic column fabrication is thoroughly discussed, paying close attention to the fine tuning options for the separation driving electroosmotic flow. Application examples of CEC in process analytical technology for the biopharmaceutical and biomarker discovery in the biomedical fields are also given.

Keywords:

• Capillary electrochromatography
• proteins
• carbohydrates
• monolithic capillary column
• open tubular capillary column

Disclosure statement

The authors declare that they have no conflict of interests.

Additional information

Funding

The authors gratefully acknowledge the support of the National Research, Development and Innovation Office (NKFIH) (K 116263) grants of the Hungarian Government, the EFOP-3.6.3-VEKOP-16-2017-00009 co-financed by EU and the European Social Found. This work was also supported by the BIONANO_GINOP-2.3.2-15-2016-00017 project and the V4-Korea Joint Research Program, project National Research, Development and Innovation Office (NKFIH) (NN 127062) grants of the Hungarian Government. This is contribution #152 from the Horváth Csaba Memorial Laboratory of Bioseparation Sciences.