Differential expression of MC1R gene in Liaoning Cashmere goats with different coat colors

Abstract

Melanocyte stimulating hormone receptor (MC1R) has been known as a regulator of eumelanin and phaeomelanin production in the melanocytes, and MC1R mutations causing coat color changes are known in many vertebrates; however, there are no research reports about the differentially expression of MC1R gene and its coding protein in Cashmere goats with different coat color. We examined the presence of MC1R distribution and MC1R protein and gene expression in the white Cashmere goats and black Cashmere goats, respectively; q-PCR, Western blot and immunhistochemical analysis showed that the expression of the MC1R gene in the black Cashmere goats was 3.39 fold more than the white ones (p < 0.01), and Cashmere goats with black genotype had significantly higher (2.03, p < 0.01) MC1R protein expression than white genotype in the all investigated samples. Moreover, all Cashmere goats with different coat color available for immunhistochemical analysis showed either lower (white Cashmere goats) or higher (black Cashmere goats) expression of the MC1R protein; these findings suggested that it had a relationship between the MC1R and the coat color of Cashmere goats. That could lay the foundation for the further research of the MC1R and coat color controllability regulation of the Cashmere goats.

Keywords:

• MC1R
• pigmentation
• Cashmere goat
• immunohistochemical

Additional information

Funding

This research was supported by the Cashmere goat breeding center of Liaoning province. And this work was supported by grants from the Natural Science Foundation of Jilin Province of China (20170101156JC), Special Funds for Scientific Research on Public Causes (201303119), and the National Natural Science Foundation of China (NSFC) (Nos. 31072097 and 30800807).