Effects of spermine on the proliferation and migration of porcine intestinal epithelial cells

Abstract

Whether spermine promotes the repair of porcine intestinal epithelium damage through Ras-related C3 botulinum toxin substrate 1 (Rac1)/phospholipase C-γ1 (PLC-γ1) signaling remains unclear. The current study investigated the effects of spermine addition on the proliferation and migration of IPEC-J2 cells and the effects of Rac1/PLC-γ1 signaling on cell migration. We showed that the inhibitors of Rac1 (NSC-23766) and PLC-γ1 (U73122) reduced cell migration and decreased the protein levels of Rac1 and PLC-γ1 in the cells. Moreover, spermine promoted the proliferation and migration of the IPEC-J2 cells, that is, 1 µM spermine exhibited the best effect, and spermine treatment increased the protein levels of Rac1 and PLC-γ1. Further experiments showed that spermine treatment increased cell migration and enhanced Rac1 and PLC-γ1 protein levels, compared with NSC-23766 and U73122 treatments with spermine. In conclusion, spermine treatment promoted the repair of damaged porcine intestinal epithelium by accelerating cell proliferation and migration mediated by Rac1/PLC-γ1 signaling.

Keywords:

• Spermine
• cell proliferation
• cell migration
• Rac1/PLC-γ1 signaling

Acknowledgments

The authors are grateful to the members of our team for their industrious assistance in this study.

Disclosure statement

There are no potential conflict of interest was declared by authors.

Additional information

Funding

This study was supported by Sichuan Science and Technology Program [No. 2020YJC0398] and the Specific Research Supporting Program for Discipline Construction in Sichuan Agricultural University [No. 03570126].