5d CIDR-Heatsynch improves the circulatory estradiol levels, estrus expression and conception rate in anestrus buffalo (Bubalus bubalis)

Abstract

The present study aimed to investigate whether increasing estradiol (E₂) during preovulatory period would increase estrous expression, luteal profiles and conception rate in 5d CIDR based timed AI protocol. A total 156 anestrus buffalo allocated (78 per group) to either 5d CIDR-Cosynch (d-5: CIDR + GnRH; d0: PGF_2α+CIDR removal; 72 h post-CIDR removal: GnRH) or 5d CIDR-Heatsynch (d-5: CIDR + GnRH; d0: PGF_2α+CIDR removal; 24 h post-CIDR removal: estradiol benzoate) group. All the buffaloes inseminated at 72 and 84 h post-CIDR removal. A subset of buffalo (n = 58) were subjected to examination of the follicle diameter and luteal profile during protocol, post-AI on days 5 and 12. The buffalo in 5d CIDR-Heatsynch had greater (p < .05) E₂ concentrations, estrus induction and increasing trend (p < .08) for conception rate (57.7% vs. 43.6%) than 5d CIDR-Cosynch. The percentage of pregnant buffalo that exhibited estrus signs was greater (p < .01) in 5d CIDR-Heatsynch than 5d CIDR-Cosynch. Positive correlation (p < .01) was observed between POF and E₂ concentrations; POF and CL diameter, CL diameter and P₄ concentrations. Estrus response and P₄ concentrations were indicators of probability of pregnancy. In conclusion, 5d CIDR-Heatsynch tended to improve conception rate. The estrus expression and P₄ concentrations (d5 and 12 post-first-AI) is the indicator of probability of pregnancy in buffalo.

Keywords:

• Buffalo
• estrogen
• follicle
• pregnancy
• progesterone

Acknowledgements

The authors are thankful to Dr. Ankit Mangotra, Dr. Sandeep Yadav and Dr. Ajit Kumar for their unconditional help during investigation.

Author contributions

Harender Singh: Conceptualization, Investigation, Writing, Analysis, Drafting of manuscript. Anand Kumar Pandey: Conceptualization, Analysis, Resources, Review and editing of manuscript. Sandeep Kumar: Investigation, Resources, Review and editing of manuscript. Gitesh Saini: Investigation, Methodology. Rakesh Duggal: Methodology, Data collection, Result analysis. Yogesh Chandrakant Bangar: Data analysis. Sandeep Kumar: Methodology, Investigation. Ravinder Saini: Data collection, Investigation. Hitesh: Data collection, Investigation.

Disclosure statement

No potential conflict of interest was reported by the author(s).