Cytotoxicity of Pheophorbide and Andrographolide Combination on MCF-7 Cancer Cell Culture

ABSTRACT

The objective of this study was to evaluate the effect of combination of pheophorbide (Thyphonium flagelliforme) and andrographolide (Andrographis paniculata) on breast cancer cell culture of MCF-7 in a laboratory study using a posttest only control group design. human dermis fibroblast (HDF) cells were used as a control. Cell culture was obtained from YARSI University biorepository. Control and experimental treatment was given to six groups for each cell culture, namely control without treatment, P8 (pheophorbide 8 μM), A48 (andrographolide 48 μM), P2A12 (combination pheophorbide 2 μM and andrographolide 12 μM), P4A24 (combination pheophorbide 4 μM and andrographolide 24 μM), and P8A48 (pheophorbide combination 8 μM and andrographolide 48 μM). The treatment was given for 24 h, with three repetitions for each group. Results showed a decrease in cell viability in all treatment groups compared to the control group both in MCF-7 cells and HDF cells. Increasing the dose did not cause an increase in toxicity to MCF-7 cells. The use of a combination of pheophorbide and andrographolide showed anti-cancer effectiveness in MCF-7 breast cancer cell lines at 4 μM pheophorbide doses and 24 μM andrographolide

KEYWORDS:

• pheophorbide
• andrographolide
• viability
• CCK-8
• mcf-7

Acknowledgment

We would like to express our gratitude to YARSI University through the Internal Research Fund for conducting this research.