Effects of in vitro culture at the preimplantation embryo stage on early development and hypertension in ISIAH rats

ABSTRACT

Objective: The study investigates how IVC and ET affect hypertensive phenotype in ISIAH rats.

Methods: ISIAH rats born as the result of ET and IVC were compared with naturally conceived controls.

Results: Embryo transfer from hypertensive ISIAH rats to normotensive recipient dams caused lower SBP and DBP in the female offspring. When ET was accompanied with in vitro culture in R1ECM, not only the female offspring, but also males demonstrated alleviation of hypertension.

Conclusions: IVC at the preimplantation embryo stage affected the manifestation of hypertension in the adult ISIAH offspring.

KEYWORDS:

• In vitro culture
• embryo transfer
• offspring neurodevelopment
• blood pressure
• hypertensive rats

Acknowledgments

The authors thank Victor Gulevich for linguistic check.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The studies are implemented using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG SB RAS, supported by the Ministry of Education and Science of Russia [Unique identifier of the project RFMEFI62117X0015]. This study was supported by budgeted project [0324‐2019‐0041] for the Institute of Cytology and Genetics – Russia.