ABSTRACT
Objective: To investigate whether the supernatant from monocytes of preeclamptic and normotensive pregnant women, cultured in vitro with silibinin, can modulate oxidative stress in HUVEC.
Methods: Concentrations of IL-1β, IL-10, and TNF-α in monocyte culture supernatants were determined by ELISA. HUVEC and their supernatant cultures were employed for determination of NO, nitrite and nitrate, lipid peroxidation, and hemeoxygenase-1 (HO-1).
Results: HUVEC treatment with supernatant of preeclamptic monocytes cultured with silibinin produced increased levels of nitrite, reduced lipid peroxidation, and increased HO-1.
Conclusion: Supernatant of monocytes from preeclamptic women induce oxidative stress in HUVEC which can be reduced by silibinin treatment.
Abbreviations: DAF-FMTM, Diaminofluorescein-FM; EDTA, Ethylenediaminetetraacetic acid; HO-1, heme oxygenase-1; HPLC, high-performance liquid chromatography; HUVEC, human umbilical vein endothelial cell; MDA, malondialdehyde; NO, nitric oxide; NT, normotensive; PE, preeclampsia; ROS, reactive oxygen species; Sb, silibinin.
Acknowledgments
The authors would like to express their gratitude to Annika Olsson and Carina Nihlén, from the Department of Physiology and Pharmacology at Karolinska Institutet for their expertise and methodological help in using the HPLC technique.
Disclosure statement
All authors report no conflicts of interest.
Author contributions
VJG, MTSP, VCS, and MC conceived and designed the study, analyzed the results, and wrote the manuscript; JCP selected individuals for the study; VJG, PRN, and SMH performed the experiments. All authors read and approved the final version of the manuscript.