Study on the relationship between SLCO1B1 and ApoE gene polymorphisms and the risk of coronary heart disease in the Mongolian population

ABSTRACT

Objective This study aimed to analyze the influence of SLCO1B1 and APOE gene polymorphisms on coronary heart disease in Mongolian population who living in Ordos area. Methods From January 2019 to June 2020, 200 Mongolian patients with coronary heart disease admitted to our hospital and other banner hospitals were selected as the case group. At the same time, 150 randomly selected healthy Mongolian people from medical examination centers comprised the control group. The polymorphisms of SLCO1B1 (388A>G, 521 T > C) and ApoE (388 T > C, 526 C > t) were detected by real-time polymerase chain reaction. Combined with environmental data, the effect of gene polymorphism on coronary heart disease was explored. Results Both SLCO1B1 and ApoE polymorphisms satisfied Hardy-Weinberg equilibrium. The SLCO1B1 genotype *1a/*1b showed the highest frequency in the case group, accounting for 35.0%, while The SLCO1B1 genotype *1b/*1b showed the highest frequency in the control group, accounting for 32.0%. Allele *1b was the most commonly seen allele in both the case group and control group (57.8% and 53.7%, respectively). Meanwhile, The difference in the distribution of SLCO1B1 *1a/*15 genotype between the two groups was statistically significant (P < .05). Conclusion The results showed that the SLCO1B *1a/*15 genotype, ApoE ε3 /ε3 genotype, and ε3 allele reduced the risk of coronary disease in the Mongolian population, making them protective genes against this disease, while the ApoE ε4 allele increased the risk of coronary disease, making it a coronary disease risk factor.

KEYWORDS:

• Mongolian
• apolipoprotein E
• gene polymorphism
• coronary disease

Acknowledgments

No funding or sponsorship was received for this study or publication of this article.

Availability of data and materials

All data generated or analyzed during this study are included in this published article

Consent for publication

The manuscript is not submitted for publication or consideration elsewhere.

Disclosure Statement

All authors have completed the ICMJE uniform disclosure form. The authors have no conflicts of interest to declare.

Ethics approval and consent to participate

The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by ethics committee of Ordos Central Hospita. After explaining the operation steps, all patients gave their informed consent.

Author Contributions

(I) Conception and design:Niu RB

(II) Administrative support: Dong XX and Guo LP

(III) Provision of study materials or patients:Pan L and Hai YQ

(IV) Collection and assembly of data:QIN C

(V) Data analysis and interpretation:Chen XX and Duan BS

(VI) Manuscript writing: All authors

(VII) Final approval of manuscript: All authors

Additional information

Funding

This work was supported by Natural Science Foundation of Inner Mongolia Autonomous Region of china[2019MS08183]; Ordos Central Hospital in-hospital subject [EY2019001].