Autoxidation and photooxidation of tetrahydrobiopterin: a theoretical study

Abstract

Pterins are naturally occurring pigments and enzyme cofactors widespread in living organisms. Tetrahydrobiopterin (H₄Bip) is a coenzyme of aromatic amino acid hydroxylases, NO-synthases, and alkylglycerol monooxygenases. This coenzyme is prone to oxidation in the presence of molecular oxygen, a so-called autoxidation. The reactions participating in H₄Bip autoxidation are well known. However, our study is an attempt to evaluate theoretically the feasibility of reactions participating in autoxidation. To do so, we have calculated the Gibbs free energy of elementary reactions between H₄Bip, its derivatives, molecular oxygen, and reactive oxygen species (ROS). In the last few years, we have established the photosensitized oxidation of H₄Bip experimentally. Thus, we have also evaluated the feasibility of H₄Bip photooxidation reactions, which may occur according to both type-I and type-II photosensitized oxidation. We calculated Fukui indices for H₄Bip and found particular atoms in the molecule that interact with nucleophiles (for example, singlet oxygen ¹O₂) and radicals (in particular, molecular oxygen ³O₂). Therefore, we evaluated the probability of H₄Bip autoxidation reactions, photooxidation reactions, and the reactivity of particular atoms in H₄Bip molecule using the theoretical methods of quantum chemistry.

Keywords:

• Tetrahydrobiopterin autoxidation
• photooxidation
• molecular oxygen
• density functional theory
• Gibbs free energy

Disclosure statement

The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Additional information

Funding

The research of A.A. Buglak and T.A. Telegina was supported by the Russian Science Foundation grant [20-73-10029].