Abstract
Background
Oxidative stress injury is an important pathological factor of premature ovarian failure (POF). Salidroside, extracted from the Chinese herb-Rhodiola rosea, has advantages in antioxidant characteristics. However, their therapeutic efficacy and mechanisms in POF have not been explored.
Purpose
This study aims to assess the therapeutic effects of salidroside in chemotherapy-induced ovarian failure rats.
Methods
A POF rat model was established by injection of cyclophosphamide, followed by treatment with salidroside. The therapeutic effect of salidroside was evaluated based on hormone levels, follicle count, and reproductive ability. Oxidative stress injury was assessed by the detection of SOD enzyme activity and MDA levels. Differential gene expression of Keap1, Nrf2, HMOX1, NQO1, AMH, BMP15, and GDF9, were identified by qRT‑PCR. The protein expression of Keap1, Nrf2, P53, and Bcl-2 were detected by western blot.
Results
Salidroside treatment markedly restored FSH, E2, and AMH hormone secretion levels, reduced follicular atresia, and increased antral follicle numbers in POF rats. In addition, salidroside improves fertility in POF rats, activates the Nrf2 signaling pathway, and reduces the level of oxidative stress. The recovery function of high dose salidroside (50 mg/kg) in a reproductive assay was significantly improved than that of lower dose salidroside (25 mg/kg). Meanwhile, the safety evaluation of salidroside treatment in rats showed that salidroside was safe for POF rats at doses of 25–50 mg/kg.
Conclusions
Salidroside therapy improved premature ovarian failure significantly through antioxidant function and activating Nrf2 signaling.
Author contributions
Lixuan Chen and Qinglin Mo contributed equally to this work. Yang Xiao and KaiXian Deng were mainly responsible for project design, and the acquisition of financial support. The experiments in rats were performed by Lixuan Chen and Yinnang Wu. Wancheng Chen and Qinglin Mo mainly assisted in the completion of experiments, and proofread the article. The manuscript was written by Lixuan Chen.
Disclosure statement
No potential conflict of interest was reported by the author(s).