Extracellular L-asparaginase production in solid-state fermentation by using sugarcane bagasse as support material

Abstract

L-asparaginase is an important enzyme used in the pharmaceutical and food industry, which can be produced by different microorganisms using low cost feedstocks. In this work, sugarcane bagasse (SCB) was used as support for enzyme production in solid-state fermentation (SSF) by A. terreus. Initially, the influence of the variables carbon and nitrogen sources on the enzyme production was studied following an experimental design carried out in Erlenmeyer flasks. Statistical analysis indicated the use of 0.54% of starch, 0% of maltose, 0.44% of asparagine, and 1.14% of glutamine in the medium, resulting in enzyme activity per volume of produced extract of 120.723 U/L. Then, these conditions were applied in a horizontal column reactor filled with SCB, producing 105.3 U/L of enzyme activity. Therefore, the potential of extracellular L-asparaginase enzyme production in the column reactor using sugarcane bagasse as support was demonstrated and it represents a system that can favor large scale production.

Keywords:

• Aspergillus terreus
• Column bioreactor
• L-asparaginase
• Solid state fermentation
• Sugarcane bagasse

Additional information

Funding

The authors gratefully acknowledge the Program PEC-PG of CAPES, FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) (grant number #2014/27055-2, #2016/10636-8 and #2017/11086-4) and CNPq (grant number #154193/2018-6) for the financial support. Also, we acknowledge Fundação André Tosello for the donation of the microorganism and Edeniq Brasil Importação e Comércio de Produtos e Servicos Ltda. for the sugarcane bagasse.