Evaluation of antioxidation, regulation of glycolipid metabolism and potential as food additives of exopolysaccharide from Sporidiobolus pararoseus PFY-Z1

Abstract

At present, there are relatively few studies on the production of exopolysaccharide (EPS) by yeasts. Therefore, exploring the properties of EPS produced by yeast can not only enrich the source of EPS, but also play an important role in its future application in the food field. The aim of this study was to explore the biological activities of EPS (named SPZ) from Sporidiobolus pararoseus PFY-Z1, as well as the dynamic changes in physical and chemical properties that occur during simulated gastrointestinal digestion, and the effects of SPZ on microbial metabolites during fecal fermentation in vitro. The results revealed that SPZ had good water solubility index, water-holding capacity, emulsifying ability, coagulated skim milk, antioxidant properties, hypoglycemic activities, and bile acid-binding abilities. Furthermore, the content of reducing sugars increased from 1.20 ± 0.03 to 3.34 ± 0.11 mg/mL after gastrointestinal digestion, and had little effect on antioxidant activities. Moreover, SPZ could promote the production of short-chain fatty acids during fermentation for 48 h, in particular, propionic acid and n-butyric acid increased to 1.89 ± 0.08 and 0.82 ± 0.04 mmol/L, respectively. Besides this, SPZ could inhibit LPS production. In general, this study can help us to better understand the potential bioactivities, and the changes in bio-activities of compounds after digestion of SPZ.

Keywords:

• Bioactivities
• exopolysaccharide
• gastrointestinal digestive
• short-chain fatty acids
• Sporidiobolus pararoseus

Disclosure statement

All authors have approved the final version of the manuscript. All authors have read and agreed to the published version of the manuscript.

Data availability statement

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

All appreciation to Qiqihar Medical University, Education Department of Heilongjiang Province, and Qiqihar Science and Technology Bureau. This research was funded by Foundation of Heilongjiang Educational Committee [2021-KYYWF-0381], Qiqihar science and technology plan joint guidance project [LSFGG-2022037], Fund project of Qiqihar Academy of Medical Sciences [QMSI 2020M-07], Foundation of Heilongjiang Educational Committee [2021-KYYWF-0349], Qiqihar science and technology plan joint guidance project [LSFGG-2022036], Fund project of Qiqihar Academy of Medical Sciences [QMSI 2022M-01].