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Abstract
Hollow fiber cell fishing (HFCF) and hollow fiber liquid phase microextraction (HF-LPME) with high performance liquid chromatography were utilized to research the anti-hepatoma HepG-2 or anti-renal tubular ACHN cells coumarins from Radix Angelicae dahuricae (RAD) in vitro and vivo. Before application, the coumarins of psoralen, bergapten, oxypeucedanin, imperatorin and isoimperatorin were selected as model compounds, the experiment conditions and methodology parameters of both HFCF and HF-LPME were investigated. Under the optimized conditions, cell growth state and survival rate, cell specific binding, repeatability for HFCF, and linear ranges, limits of quantification (1.3–21.0 ng/mL), precisions (<15.2%), recoveries (80.4%–109.3%) and stability for HF-LPME were achieved. Results showed that the five model compounds and xanthotoxol, xanthotoxin were screened in RAD extraction; xanthotoxin and xanthotoxol were fished by HepG-2 cell from both plasma and liver, xanthotoxol, xanthotoxin, bergapten and imperatorin were fished by ACHN cell from plasma and kidney; and these coumarins maintained a certain concentration in vivo. The study results provide experimental basis for researching the active anticancer components and expound the medicinal material base in RAD. The combination of HFCF and HF-LPME is simple and effective in studying bioactive anticancer compounds in traditional Chinese medicine in vitro and in vivo.
Graphical Abstract
Disclosure
The author confirm that this article content has no conflict of interest.