Benzo[a]pyrene constrains embryo development via oxidative stress induction and modulates the transcriptional responses of molecular biomarkers in the marine medaka Oryzias javanicus

Abstract

Embryos from the marine medaka fish Oryzias javanicus were treated with eight concentrations of benzo[a]pyrene (BaP) (0.001, 0.01, 0.1, 1, 2, 5, 10, or 20 μg L^-–1) after they had been fertilized. Significant mortality and hatching delays were detected in embryos that had been exposed to 10 and 20 μg L⁻¹ BaP for 4 weeks. The mortality rate after hatching was higher in the medaka that had been previously exposed to > 2 μg L⁻¹ BaP. Significant elevations in intracellular reactive oxygen species and malondialdehyde contents were measured and the mRNA expressions of the antioxidant defense system genes (gst, sod, cat, and gpx) increased in the embryos exposed to 10 and 20 μg L⁻¹ BaP for 1 week. The hsp70, ahr, and cyp1a transcriptional responses were also significantly upregulated in the exposed groups after 1 week. The alterations to the in vivo parameters and molecular components suggested that waterborne BaP had a toxic effect on marine medaka embryos. Finally, fin defects, spinal curvature, and cardiac edema were highly induced when the embryos were exposed to > 5 μg L⁻¹ BaP.

Keywords:

• Benzo[a]pyrene
• marine medaka
• growth parameters
• oxidative stress
• biomarker

Additional information

Funding

This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education (grant no. NRF-2017R1A6A1A06015181).