Homoploid hybrids of Origanum (Lamiaceae) in Turkey: morphological and molecular evidence for a new hybrid

Abstract

In this study, three hybrids of Origanum (Lamiaceae) were examined in detail using morphological, karyological, palynological and molecular approaches. One of the three hybrids, O. × aytacii, is here described for the first time, and is compared with its putative parents, O. sipyleum and O. vulgare subsp. hirtum. In addition to morphological, karyological and palynological features, we also examined nuclear ribosomal DNA (ITS) sequences of O. × intermedium (O. onites × O. sipyleum), O. × intercedens (O. onites × O. vulgare subsp. hirtum) and their purported parental species that share the same area with the new hybrid. Pollen grains were hexacolpate and isopolar. The range of Polar/Equatorial ratio (P/E) of pollen for investigated taxa varied from 0.74 to 0.99. All investigated taxa are diploid with a chromosome number as 2n = 30. According to molecular results, O. × aytacii was the most polymorphic taxon. In addition, DNA sequences showed that O. × intercedens was more similar to O. vulgare subsp. hirtum than to O. onites, and O. × intermedium was more similar to O. onites than to O. sipyleum. Furthermore, O. onites and O. vulgare subsp. hirtum have many different ITS haplotypes, probably resulting from backcrossing with different individuals. Results obtained in this study demonstrate that hybridization has played a major role in the process of Origanum speciation.

Keywords:

• Hybridization
• homoploidy
• Origanum
• Origanum × aytacii
• pollen
• ITS
• Turkey

Acknowledgements

We would like to thank Assoc. Prof. Dr. Ferhat Celep and Dr. Bryan Drew for their valuable comments to manuscript and Serap SEMERCİ (English teacher) for checking the manuscript in terms of language, and the curators of the following herbaria, which gave us permission to examine the specimens: ANK, B, BM, E, EGE, G, GAZI, GOET, HUB, ISTE, ISTF, K, LE, W, and WU.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

We would like to thank TÜBİTAK (Project No: 113Z225) for financial support for our research.