Phytohormone involved in salt tolerance regulation of Elaeagnus angustifolia L. seedlings

ABSTRACT

Soil salinization is a serious problem for plant growth worldwide. Understanding the tolerance mechanisms of salt-tolerant plants is essential for development and utilization of saline-alkali land. The salt-tolerant tree species Elaeagnus angustifolia is utilized in landscaping and ecological restoration projects such as afforestation. In this work, we examined the changes in phytohormone levels and the expression of phytohormone-related genes in E. angustifolia seedlings under salt stress. We observed no changes in the biomass, leaf area, and the size of epidermal cells of E. angustifolia seedlings under low levels of NaCl (50 mM); however, when the NaCl concentration was increased, these parameters were significantly reduced. Comparison of the transcriptome data of the species seedlings under 0 and 150 mM NaCl showed that many hormone pathways are involved in the salt-tolerance response. Under salt stress, abscisic acid (ABA) and jasmonate (JA) contents increased significantly, whereas the contents of auxin (IAA), gibberellin (GA), cytokinins (CKs), and brassinosteroids (BRs) decreased significantly. We conclude that these changes could be responsible for the growth inhibition and increased salt resistance of E. angustifolia seedlings.

KEYWORDS:

• Elaeagnus angustifolia L.
• NaCl stress
• plant hormones
• transcriptome
• hormone balance

Authors’ Contribution Statement

Tingting Zhu and Jing Lin wrote this manuscript; Tingting Zhu and Jing Lin performed experiments; Mingjing Zhang, Lingyu Li and Chen Zhao collected data, carried out all analyses; Min Chen conceptualized the idea. All authors read and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was supported by Shandong Natural Science Research Foundation of China [ZR2019MC065], the major projects of science and technology in Shandong province [2017CXGC0311, 2017CXGC0313] and the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province.