Hepatoprotective effects of ethyl pyruvate against carbon tetrachloride-induced oxidative stress, biochemical and histological alterations in rats

Abstract

This study investigated the protective effects of ethyl pyruvate (EP) against carbon tetrachloride (CCl₄)-induced acute hepatic injury in rats. The administration of a single dose of CCl₄ (1.6 g/kg body weight) significantly elevated the levels of malondialdehyde, nitric oxide, alanine transaminase, aspartate transaminase, and alkaline phosphatase, cholesterol, low-density lipoprotein cholesterol, and triglycerides. In addition, CCl₄ was found to significantly suppress the activity of superoxide dismutase, catalase, and glutathione peroxidase. All of these parameters were restored to their normal levels by the administration of EP before and after the CCl₄ injection. Moreover, the number of positive apoptotic hepatocytes had significantly increased in the CCl₄ group but decreased in rats treated with EP along with CCl₄. Histopathological changes induced by CCl₄ were also ameliorated by EP treatment. These findings provided evidence that EP, because of its antioxidant and anti-apoptotic action, could protect rat liver against CCl₄-induced acute liver injury.

Keywords:

• Antioxidant
• apoptosis
• carbon tetrachloride
• ethyl pyruvate
• liver injury

Disclosure statement

The authors have all disclosed no conflicts of interest.

Additional information

Funding

This research was supported by the Research Fund of Erciyes University (Project code: TSY-113606).