Abstract
Objectives
Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population.
Methods
Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer, 10 patients with COPD and 10 healthy volunteers. we examined their expression in 10 bronchoalveolar lavage samples and assessed correlation with the serum levels.
Results
There was an inverse relationship between expression of serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 (r = −0.62, p = .00). Both serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 showed a significant positive and negative association with lung cancer patients respectively in comparison to patients with COPD and healthy persons (p < .001).
Conclusion
RAB27A mRNA and Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer.
Ethical approval and informed consent
Written informed consent was obtained from all the participants of this study, which was performed in accordance with Declaration of Helsinki, and was approved by the Ethics Committee of Ain Shams Faculty of Medicine, Egypt. This work was carried out in compliance with Ethical Standards.
Disclosure statement
All the authors; Ghada Mohamed Gamal El-Din, Fawzia Khalil Ibrahim, Hanan Hussein Shehata, Nehad Mohammed Osman, Omar Mohammed Abdel-Rahman and Marwa Ali, declared that they have no competing interest.