Phenolic composition, antioxidant and antinociceptive activities of Syringa vulgaris L. bark and leaf extracts

Abstract

Metabolite profile, antioxidant and antinociceptive activities of Syringa vulgaris bark and leaf methanolic extracts were investigated. By means of HPLC-DAD-ESI-TOF and HPLC-DAD-ESI-MS/MS, a total of 33 phenolics were identified, including 15 secoiridoids, 6 phenylpropanoids, 3 flavonoids, 3 lignans and 6 low molecular weight phenols. Validated quantitative analysis show that syringin (2.52%) and rutin (1.13%) are the main phenolic compounds in bark and leaf, respectively. Notable radical scavenging and antinociceptive activities of the bark and leaf extracts were confirmed by in vitro DPPH^● and ABTS^●+ assays and by in vivo hot-plate method in mice, respectively. Our results could lay the scientific basic of future clinical perspectives of lilac bark and leaf.

Keywords:

• Phenolic profiling
• Syringa vulgaris
• oleuropein
• antioxidant activity
• antinociceptive activity

Acknowledgements

This work was supported by the Transylvanian Museum Society and Semmelweis University research grant number 485/P.2.EMEOGYSZ, 2014. The financial support from ÚNKP-17-4 New National Excellence Program of the Ministry of Human Capacity is highly appreciated (G.T).