Cytotoxic components from the Xisha sponge Fascaplysinopsis reticulata

Abstract

A new dolabellane diterpenoid, clavirolide H (1), together with eleven known compounds, including two dolabellane diterpenoid (2 and 3), a rare cavernosine-type C₁₇ γ-lactone terpenoid (4), a diketopiperazine (5) and seven sterols (6–12), were isolated from the Xisha sponge Fascaplysinopsis reticulata. Their structures were elucidated by extensive spectroscopic analysis, and the four types of compounds of the above isolates were reported from the genus Fascaplysinopsis for the first time. Selected compounds 1, 4–6 and 9–12 were evaluated for cytotoxic activities against K562, HL-60, Hela, HCT-116, A549, L-02 and BEL-7402 cell lines. Compounds 4–6 and 10–12 showed potent cytotoxicitives against HL-60 with IC₅₀ values ranging from 8.8 to 12.4 μM. Compounds 4 and 5 exhibited weak cytotoxic activities against HeLa with IC₅₀ of 20.7 and 27.4 μM, and 5 also has moderate cytotoxicity against HCT-116 with IC₅₀ of 16.3 μM.

Keywords:

• Sponge
• Fascaplysinopsis reticulata
• dolabellane diterpenoid
• cavernosine-type C17 γ-lactone terpenoid
• diketopiperazine
• sterols
• cytotoxicitives

Acknowledgements

Special thanks are given to Prof. Jing Li for the cytotoxicities tests.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This work was supported by the National Natural Science Foundation of China [grant numbers 41476107, 41522605], AoShan Talents Program Supported by Qingdao National Laboratory for Marine Science and Technology [grant number 2015ASTP], and the Shandong Natural Science Fund for Distinguished Young Scholars [grant number JQ201606].