A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical

Abstract

A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC₅₀ values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC₅₀ 5.45 µM) and sucrase (IC₅₀ 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC₅₀ values 1.92 and 5.58 mM.

Keywords:

• Clausena excavata
• pyrano coumarin
• inhibition
• α-glycosidase
• antioxidant
• IC50

Acknowledgement

T.M.T. would like to thank Universitas Airlangga, Directorate General of Higher Education (DIKTI), Ministry of Education and Culture of Indonesia that has funded this research.

Disclosure statement

No potential conflict of interest was reported by the authors.