Vitexnegheteroin M, a new phenolic glycoside from Vitex negundo var. heterophylla

Abstract

The investigation of chemical constituents from the leaves and seeds of Vitex negundo var. heterophylla resulted in the isolation of one new phenolic glycoside, vitexnegheteroin M (1), together with eleven known compounds (2-12). Their structures were elucidated through extensive spectroscopic analyses including 1D, 2D NMR, HR-ESI-MS, and by comparison with the literature. Among them, compound 3 was obtained from the Vitex genus for the first time, and compounds 4-8, and 10-12 were isolated from this plant for the first time. Their anti-inflammatory activities were investigated by measuring nitric oxide (NO) production in lipopolysaccharide (LPS)-induced murine microglial BV-2 cells. As the obtained results, compound 9 showed the strongest inhibitory activity against LPS-stimulated NO production in BV-2 cells with IC₅₀ value ranging from 8.65 ± 0.67 μM.

Keywords:

• Vitex negundo var. heterophylla
• phenolic glycoside
• anti-inflammatory activity
• NO production

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Natural Science Foundation of Liaoning Province (20180550204), Scientific Research Foundation of Shenyang Medical College (20191022), and Undergraduate Scientific Research Projects of Shenyang Medical College (20189025).