Rosa chinensis in vitro cell cultures: a phytocomplex rich of medium molecular weight polysaccharides with hydrating properties

Abstract

In-vitro cell cultures of selected Rosa chinensis meristematic cells cultivated with an innovative CROP^® (Controlled Release of Optimized Plants) platform, allowed obtaining a stable and standardized phytocomplex rich of medium molecular weight polysaccharides. The polysaccharides profile of the rose extract has been analysed with the size exclusion chromatography (HPLC-ELSD-SEC) both in the in vitro extract and in the dried petals of Rosa chinensis. The polysaccharides content in the extract was ≥20%, higher than in the dried petals. The 65–80% of total polysaccharides have a medium molecular weight (1000 Da), known for their moisturizing and anti-age properties. Reconstructed human epidermis in homeostatic conditions was used to evaluate its moisturizing action and the ability to maintain homeostasis. The Rosa chinensis extract increased the Aquaporin-3 expression and cell membrane localization and demonstrated to regulate hydration either in topical and systemic exposure.

Graphical Abstract

Keywords:

• Rosa chinensis
• in vitro cell culture
• phytocomplex
• polysaccharide
• aquaporin-3
• reconstructured human epidermis

Acknowledgements

Thanks to all technical team of Demethra biotech Srl for the support in the development of Rosa chinensis cell line.

Disclosure statement

No potential conflict of interest was reported by the authors.