Leaf-surface guaianolides from Amphoricarpos neumaeyri showing protective effect on human lymphocytes DNA

Abstract

Analysis of composition of CH₂Cl₂ surface extract of leaves of Amphoricarpos neumayeri Vis. revealed 16 sesquiterpene lactones with guaianolide skeleton, so called amphoricarpolides, typical for this genus. Four of them, 13–16, were new derivatives and their structures were elucidated by detailed analyses of IR, NMR and MS data. Amphoricarpolide (9), its 15-O-acetyl derivative (5), and two 9β-hydroxyamphoricarpolides, 3,15-di-O-acetyl- and 3-O-acetyl-15-O-isovaleroyl derivatives (3 and 6, respectively) were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The tested compound exerted a beneficial effect by decreasing DNA damage of human lymphocytes.

Graphical Abstract

Keywords:

• Amphoricarpos neumayeri Vis.
• sesquiterpene lactones
• in vitro protective effect on chromosome aberrations

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was supported by the Ministry of Science, Education and Technological Development of Serbia (grant no. 172053), as well as the Serbian Academy of Sciences and Arts (project F-188).