In vitro antitubercular, anticancer activities and IL-10 expression in HCT-116 cells of Tinospora sinensis (Lour.) Merr. leaves extract

Abstract

Considering the importance of Tinospora sinensis in folklore as immunomodulant, the leaves subjected to cold maceration using ethanol, ethyl acetate and n-hexane and then screened for their antitubercular, anticancer and IL-10 expression (HCT-116) properties using luciferase reporter phage, MTT and flow cytometry assay methods, respectively. The results revealed that ethyl acetate and n-hexane extracts demonstrated the promising (p < 0.05) antitubercular activity with inhibition (%) of 92% and 86%, at 100 µg/ml, respectively and the respective IC₅₀ values of 63.99 and 113.7 µg/ml on HeLa and HCT-166 cells. The n-hexane extract demonstrated the significant (p < 0.05) IL-10 expression (44.12 MFU) in HCT-116 cells against the control cells (8.48 MFU). The GC-MS analyses of n-hexane extract showed presence of tembatarine, berberine, cordifoliside E, and magnoflorine as major constituents. Among all extracts, n-hexane leaf extract was found to be potent against inhibition of colon cancer cells (HCT-116) and Mycobacteria, with significant immunostimulant activity.

Graphical Abstract

Keywords:

• Tinospora sinensis
• anticancer
• antitubercular
• immunomodulatory
• IL-10
• HeLa
• MCF-7
• HCT-116

Acknowledgment

Authors are thankful to DST-FIST Facility of Raghavendra Institute of Pharmaceutical Education and Research, Anantapur, AP, India and ICMR-National Institute of Research in Tuberculosis (NIRT), Chennai, Tamil Nadu, and India for the support provided.

Disclosure statement

Authors have no conflict of interest.