A novel peptide from the skin of amphibian Rana limnocharis with potency to promote skin wound repair

Abstract

In clinical trials, the healing of wounds remains a substantial physiological and financial incumbrance on patients. Therefore, the development of new drugs that can accelerate wound healing is vital. Based on genomic methods, we identified a new peptide (RL-RL10) with the amino acid sequence ‘RLFKCWKKDS’ from the skin of an amphibian frog species (Rana limnocharis). RL-RL10 promoted wound healing of human keratinocytes (HaCaT) in a concentration-dependent manner. RL-RL10 also had an effect on the migration and proliferation of HaCaT cells and promoted healing of a full-thickness wound in mice in a dose-dependent manner. In conclusion, we discovered RL-RL10 that promoted healing activity of cellular and animal wounds, thus providing a new peptide template for the development of novel wound-repairing drugs.

Graphical Abstract

Keywords:

• Rana limnocharis
• skin secretions
• human keratinocytes
• wound healing
• peptide
• RL-RL10

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants from the Chinese National Natural Science Foundation (81760648, 31670776 and 31460571) and Yunnan Applied Basic Research Project Foundation (2017FB035 and 2019FB128).