Cytotoxicity and antibacterial activity of scutellarein and carajurone-enriched fraction obtained from the hydroethanolic extract of the leaves of Fridericia chica (Bonpl.) L.G. Lohmann

Abstract

The hydroethanolic extract obtained from the dry leaves of Fridericia chica (HEFc) underwent several fractionations by different chromatographic techniques. The ethyl acetate and dichloromethane fraction were subjected to phytochemical analysis, resulting in the identification and isolation of scutellarein (1) and in a fraction rich in carajurone (2). They were tested for cytotoxicity in CHO-K1 and the antibacterial activity and mode of action by in vitro assays. The HEFc and scutellarein (1) presented no cytotoxicity. The results showed good antibacterial effect of HEFc against Streptococcus pyogenes and Staphylococcus aureus and moderate activity for Staphylococcus epidermidis, Pseudomonas aeruginosa and Salmonella typhimurium. The fraction containing the compound carajurone (2) showed good activity against Staphylococcus aureus and Staphylococcus epidermidis and moderate activity against Streptococcus pyogenes. Scutellarein (1) showed no activity against the bacteria tested. HEFc antibacterial mode of action appeared to be associated with changes in the permeability of bacterial membranes and nucleotide leakage.

Graphical Abstract

Keywords:

• Fridericia chica
• scutellarein
• carajurone
• antibacterial

Disclosure statement

The authors declare no conflict of interest.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This study was financially supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Pró-Amazônia, Proc. no. 23038.000731/2013-56), ‘Conselho Nacional de Desenvolvimento Científico e Tecnológico’ (CNPq/BIONORTE, Proc. no. 551737/2010-7), ‘Fundação de Amparo à Pesquisa do Estado de Mato Grosso’ (FAPEMAT, Proc. no. 205978/2011) and to ‘Instituto Nacional de Ciência e Tecnologia em Áreas Úmidas’ (INAU-INCT-MCTI/CNPq/CAPES/FAPs no. 16/2014). Karuppusamy Arunachalam is recipient of National Post-Doctoral Fellowship (CAPES/PNPD, Proc. no. 23108.180072/2016-02).