Anti-inflammatory ergosterol derivatives from the endophytic fungus Fusarium chlamydosporum

Abstract

Two new ergosterol derivatives namely, chlamydosterols A [(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol 6-decanoate] (1) and B [5α,6β,25-trihydroxy-(22E,24R)-ergosta-7,22-dien-3-one] (5) and three known ergosterols: ergosta-7,22-dien-3β-ol (2), ergosta-5,7,22-triene-3β-ol (3), and ergosta-7,22-diene-3β,5α,6β-triol (4) were separated from the EtOAc extract of the endophytic fungus Fusarium chlamydosporum isolated from Anvillea garcinii (Asteraceae) leaves growing in Saudi Arabia. Their structural assignment was accomplished by various spectroscopic analyses, as well as comparing with the published data. The 5-lipoxygenase (5-LOX) inhibitory potential of the isolated metabolites was assessed. Compounds 1 and 3 displayed moderate 5-LOX inhibitory potential with IC₅₀s 3.06 and 3.57 µM, respectively compared to indomethacin (IC₅₀ 1.13 µM).

Graphical Abstract

Keywords:

• Fusarium chlamydosporum
• Anvillea garcinii
• Asteraceae
• Endophytes
• ergosterol derivatives
• anti-inflammatory

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, under grant no. (G- 1725- 140- 1440). Therefore, authors, acknowledge with thanks DSR for technical and financial support.