Investigation of antihyperalgesic and antiedematous activities of three Hieracium species

Abstract

Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED₅₀=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED₅₀=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.

Graphical Abstract

Keywords:

• Hieracium
• triterpene/polyphenol rich extracts
• carrageenan-induced inflammation
• antihyperalgesic activity
• antiedematous activity
• acute toxicity screening

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This research was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia under Grant (No. 175045) and Grant (No. 173021).