Chemical constituent from the peel of Trichosanthes kirilowii Maxim and their NF-κB inhibitory activity

Abstract

The peel of Trichosanthes kirilowii Maxim is clinically used to treat cardiovascular diseases in China. In this study, the NF-κB inhibitory activity of the peel of T. kirilowii Maxim extracts was determined by Dual-Luciferase Reporter Assay System, and the results showed 70% ethanol extract could significantly inhibit the activation of NF-κB (p < 0.001). Further, 21 compounds were isolated from 70% ethanol extract. One new compound, namely (2 R)-(2-amino-2-hydroxymethyl-3-[(4-hydroxy-3-methoxybenzoyl)-O-]-propanoic acid (1), and 20 known compounds were elucidated by comprehensive spectroscopic analyses. The isolated compounds were tested in the anti-inflammatory assay, and the results indicated compounds 5, 8, 11, 14, 15, 17 and 21 could inhibit the activation of NF-κB (p <0 .05, p < 0.001) at concentration of 1 μM.

Graphical Abstract

Keywords:

• Trichosanthes kirilowii Maxim
• Trichosanthes Pericarpium
• component identification
• anti-inflammatory
• NF-κB
• dual-luciferase reporter assay system

Acknowledgements

We thank College of Pharmacy NanKai University for providing the plasmid and HEK293 cells.

Disclosure statement

All authors declare that there is no conflict of interest associated with this work.

Additional information

Funding

This work was supported partially by the National Science and Technology Major Projects for New Drug Development of China [Grant No. 2018ZX09735-002], the Tianjin Research Program of Application Foundation and Advanced Technology [Grant No. 19JCYBJC28500] and the National Natural Science Foundation of China [Grant No. 81973792].