Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component

Abstract

The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo. T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features. TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21. FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered. Combining ¹H NMR, ¹³C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.

Keywords:

• Toona sinensis (A. Juss.) Roem
• Meliaceae
• polysaccharide
• immunomodulatory activity
• characterization

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was supported by Shandong Science and Technology Research Project of Traditional Chinese Medicine (2015227), Natural Science Foundation of Shandong Province (ZR2018MH040), and National Nature Science Foundation of China (81274049).