Identification and antigiardial activity of biocompounds produced in the Ganoderma lipsiense mycelium in submerged fermentation

Abstract

Natural antiparasitic agents are used as a strategy for advances in the treatment of neglected tropical diseases (NTDs), such as giardiasis. The present paper identified the compounds produced by Ganoderma lipsiense mycelium grown in submerged fermentation and investigated the antigiardial activity potential of extracts in vitro. G. lipsiense mycelium crude extract (CE) was obtained by the maceration process and after purification in column, thirty compounds were identified by gas chromatography - mass spectrometry (GC-MS) in hexane sub-fraction (HSF). To determine the antigiardial potential, different parts of the purified CE have been evaluated, where hexane (HEXf) and dichloromethane (DCMf) fractions showed in vitro reduction levels higher than 90% of the Giardia duodenalis trophozoites at 100 μg mL⁻¹. This antigiardial activity was investigated aiming to explore the possibility of G. lipsiense mycelium extract as a novel antigiardial compound to the treatment of human giardiasis.

KEYWORDS:

• Ganoderma lipsiense
• fatty acids
• steroids
• antigiardial
• Giardia duodenalis

Acknowledgements

The authors are grateful to the Coordination for the Improvement of Higher Education Personnel - (CAPES) Brazil - for financial support (Code 001). The authors D. de Oliveira and L.B.B. Tavares are fellowship holders of the National Council for Scientific and Technological Development (CNPq).

Disclosure statement

No potential conflict of interest was reported by the author(s).