Chemical constituents and dereplication study of Lessingianthus brevifolius (Less.) H.Rob. (Asteraceae) by UHPLC-HRMS and molecular networking

Abstract

Chemical investigation of Lessingianthus brevifolius (Less.) H.Rob. aerial parts resulted in the isolation of the hirsutinolide-type sesquiterpene lactones piptocarphol, spicatolide D, piptocarphin D and 8α-acetoxy-10α-hydroxy-13-O-methylhirsutinolide, and also of a cadinanolide identified as 13-O-methylvernojalcanolide 8-O-acetate. Flavonoids, triterpenes and chlorogenic acids were also isolated. In addition, a dereplication study was carried out using UHPLC-HRMS and molecular networking, resulting in the identification of fifteen known compounds, being two sesquiterpene lactones and thirteen flavonoids. Some of the compounds are being described for the first time in L. brevifolius, and also in the Lessingianthus genus.

Graphical Abstract

Keywords:

• Asteraceae
• Lessingianthus brevifolius
• sesquiterpene lactones
• flavonoids
• dereplication
• molecular networking

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors would like to thank CAPES and CNPq (Process number 425938/2018-2) for financial support. This work was supported by the Complexo de Central de Apoio à Pesquisa (COMCAP) from the Universidade Estadual de Maringá (UEM).