Assessment of antiviral activity and mechanism of rhein on newcastle disease virus (La Sota strain IV) in vitro

Abstract

Current research is focused on the development of drug candidates from natural products. Rhein a Traditional Chinese Medicine (TCM) from Polygonaceae (rhubarb) has exhibited antioxidant, anti-inflammatory and anticancer activities, however no work has reported its antiviral potential, thus this study was performed to investigate the antiviral activities of rhein on new castle disease virus (NDV) in vitro.

NDV infection of chicken embryo fibroblasts (CEFs) was prepared using 10-day-old specific pathogen free chicken embryos. Cytotoxicity and anti-viral activities of rhein were assessed using the MTT method. The interaction between NDV and cell membrane proteins were also detected using virus overlay protein binding assay (VOPBA). In addition NDV genes expressions in CEFs were measured using real-time fluorescent quantitative (RTFQ) PCR.

The results showed that rhein effectively inhibit NDV activities maximal safe concentration of 0.125 mg/ml. This finding indicated that, rhein could be used as future antiviral drug against NDV.

Keywords:

• Chicken embryo fibroblasts
• newcastle disease
• traditional Chinese medicine
• rhein

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This research was financially supported by grants from the Science and Technology Support Program of Sichuan Province (Grant No. 2015SZ0201 and 20ZDYF1246).